RNA

Part:BBa_K5055005:Design

Designed by: Laurine Wachspress   Group: iGEM24_Ionis-Paris   (2024-10-01)


small hairpin RNA phytoene desaturase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 211
    Illegal XbaI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 211
    Illegal NotI site found at 7
    Illegal NotI site found at 203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 211
    Illegal XbaI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 211
    Illegal XbaI site found at 16
    Illegal XbaI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

At the end of our sequence, we added the Origin of Assembly Sequence to allow the encapsidation of the shRNA in the viral capsid, as we wanted for the lhRNA.


Source

This part corresponds to a fragment of the phytoene desaturase of Beta vulgaris. Its sequence was found on NCBI (NCBI Reference Sequence: XM_048641752.2).

References